therefore being studied. Our dedicated information section provides allows you to learn more about MDPI. The regulation mechanisms of AhR by molecular chaperone complex. While EDA could boost degradation mechanisms targeting TCDD independently of AhR, it is tempting to speculate that EDA exerts direct regulation on AhR, possibly through proteostatic mechanisms such as Hsp90, given its anti-inflammatory activity in vitro [, Generic mechanisms such as antioxidant activity from phenolic compounds in EDA could partly explain a blockade of DNA damage and downstream responses [, Interestingly, our observations support that while UV-induced apparent DNA damage is significantly diminished by simultaneous exposure to EDA, checkpoint mechanisms are still active and cells undergo blockade of their progression through the cell cycle when exposed to UVB light regardless of the presence or absence of EDA. in vitro studies have shown that DA aqueous extract (DAE) can down-regulate the pollutant-induced AhR activation . Deschampsia antarctica (Edafence®, EDA), a natural aqueous extract, has shown keratinocyte and fibroblast cell protection effects against ultraviolet radiation and dioxin toxicity. Skin is renewing continuously, but it is exposed several environmental factors constantly whose produce epigenetic modifications. Please let us know what you think of our products and services. The statements, opinions and data contained in the journals are solely ; Methodology, A.Z. Characterization of sunburn cells after exposure to ultraviolet light. Subscribe to receive issue release notifications and newsletters from MDPI journals, You can make submissions to other journals. International Journal of Molecular Sciences. Exposure to natural and artificial light and environmental pollutants are the main factors that challenge skin homeostasis, promoting aging or even different forms of skin cancer through a variety of mechanisms that include accumulation of reactive oxygen species (ROS), engagement of DNA damage responses, and extracellular matrix (ECM) remodeling upon release of metalloproteases (MMPs). ; Martinho, H.; Daghastanli, N.; Nantes, I.L. Active components of the antineoplastic extract are also disclosed and a method to prevent proliferation of cancerous or tumorous cells with the extract or with components thereof is disclosed. Picture processing was performed with Photoshop Extended CS5 12.0 (Adobe Systems Inc., Mountain View, CA, USA). Briefly, dry green leaves, harvested from cultured Deschampsia antarctica plants in defined conditions, were milled and extracted by percolation with water at 40–60°C, during 4–6 hours. Find support for a specific problem on the support section of our website. ; Simon, M.C. In this regard, we studied the protective capacity of EDA against the deleterious e ects of J. Mol. Liu, L.; Wise, D.R. Statistical significance was determined by statistical test of analysis of variance (ANOVA) or two-way ANOVA and Bonferroni post hoc tests, using GraphPad Prism 5.00 (GraphPad Software, Inc., San Diego, CA, USA). 2.1. Deschampsia antarctica je jeden z pouhých dvou druhů krytosemenných rostlin, jež se vyskytují v antarktické oblasti. Sinha, R.P. Ozone are agonist of AhR [10]. Deschampsia antarctica extract (EDAFENCE[R] hereinafter called EDA) was obtained from IFC, Madrid, Spain. Microscopic images were obtained using an epifluorescence microscope coupled to a CCD camera DP70 (Olympus BX-61, Tokyo, Japan) with UV filters for the excitation light (360–370 nm excitation filter UG-1), blue (450–490 nm excitation filter BP 490), or green (570–590 nm excitation filter 590 DM). 2019. Hypoxic reactive oxygen species regulate the integrated stress response and cell survival. Matrix-degrading metalloproteinases in photoaging. These authors contributed equally to this work. Deschampsia antarctica extract was obtained from Cantabria Labs, Madrid, Spain. Antioxidant Responses Induced by UVB Radiation in Deschampsia antarctica Desv. S.R.L. Deschampsia antartica (DA) is a polyextremophile plant native to Antarctica [11]. Their activity is tightly regulated by endogenous inhibitors the tissue inhibitor of metalloproteinases (TIMPs). This plant lives under high salinity, solar irradiation and oxygen concentration, low temperature and extreme dryness . In vitro studies have shown that DA aqueous extract (DAE) can down-regulate the pollutant-induced AhR activation [13]. For the detection of nuclear AhR and laminin B (Dallas, TX, USA), the extract of the cytoplasmic and nuclear proteins was performed separately with the Nuclear Extraction Kit (Merk) according to manufacturer’s instructions. Distinct Role of Sesn2 in Response to UVB-Induced DNA Damage and UVA-Induced Oxidative Stress in Melanocytes. 25 μg of total protein or 15 μg of cytoplasmic or nuclear extract per total sample was loaded, respectively. There are no exposure data on these product types. Enjoy the videos and music you love, upload original content, and share it all with friends, family, and the world on YouTube. Oxid Med Cell Longev. The present disclosure provides a novel antineoplastic extract obtained from Deschampsia antarctica plant.Active components of the antineoplastic extract are also disclosed and a method to prevent proliferation of cancerous or tumorous cells with the extract or … Liang, C.; Feng, P.; Ku, B.; Oh, B.H. ; Budanov, A.; He, Y.Y. These authors contributed equally to this work. The sources of UVA and UVB lights were a lamp of 300–400 nm and 15.9 W/m. Ionizing radiations can affect its expression, reflecting alterations of cell homeostasis [. Quan, T.; Qin, Z.; Xia, W.; Shao, Y.; Voorhees, J.J.; Fisher, G.J. Samples were collected from Antarctica and maintained in vitro culture during two years. Functions of poly(ADP-ribose) polymerase (PARP) in DNA repair, genomic integrity and cell death. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists. An extract from the plant Deschampsia antarctica protects fibroblasts from senescence induced by hydrogen peroxide. Day, T.A. High throughput sequencing showed that up to 92% of DNA reads, and 68% of RNA reads, in soils from the islands were accounted for by the fungus. Sci. For that, cells 24 h after the treatments, were incubated with MTT (50 μg/mL) for 3 h. The formazan crystals were dissolved with DMSO and the optical density was determined in a SpectraFluor plate reader (Tecan Trading AG, Switzerland) at a wavelength of 542 nm. We use cookies on our website to ensure you get the best experience. The present disclosure provides a novel antineoplastic extract obtained from Deschampsia antarctica plant. Both percentages of positive cells and mean fluorescent intensity for specific antibodies were determined by using the Image J analysis software. Different concentrations of TCDD (15–300 nM) were tested on human dermal fibroblasts and keratinocytes at different incubation times, always without exceeding a final DMSO concentration of 0.1% (Sigma, Saint Louis, MO, USA). ; Ruhland, C.T. Abstract. You seem to have javascript disabled. ; Pinto, S.M. and M.M. Ramirez, M.L.G. and S.G. Deschampsia antarctica Desv. Inhalation Toxicity: Not determined. Find support for a specific problem on the support section of our website. Deschampsia antarctica (Edafence ®, EDA), a natural aqueous extract, has shown keratinocyte and fibroblast cell protection effects against ultraviolet radiation and dioxin toxicity. The methods for obtaining the extract have been previously published . Cell viability in HaCaT and HDF cells after different treatments was determined by MTT assay. We report a previously undescribed member of the Helotiales that is superabundant in soils at two maritime Antarctic islands under Antarctic Hairgrass (Deschampsia antarctica Desv.). ; Xiong, F.S. Ultraviolet ionizing radiation (UVR) constitutes ~10% of total sunlight output, and is the most prominent causative factor for skin cancers (reviewed in open online resources: In. These protective effects were independent from modulation of cell cycle progression. Different factors derived from modern lifestyles (increased age expectancy; promotion of outdoor activity and travelling to different latitudes; increasing presence of environmental contaminants with toxic potential) challenge skin health and require the development of technologies that minimize their impact. Specifically, analysis of unsynchronized cell cultures revealed that UVB light elicited an accumulation of cells in S and G2/M phases, and simultaneous exposure to EDA did not significantly diminish this effect. Ultraviolet A radiation is the predominant component of sunlight causative of photoaging, while ultraviolet B light is considered a potentiator of photoaging. For irradiation, cell culture medium was replaced by PBS in all cases. Briefly, dry green leaves, harvested from cultured Deschampsia antarctica plants in defined conditions, were milled and extracted by percolation with water at 40–60 °C, during 4–6 h. The extract was filtered through a 1μm filter and … The new aryl hydrocarbon receptor antagonist E/Z-2-benzylindene-5,6-dimethoxy-3,3-dimethylindan-1-one protects against UVB-induced signal transduction. ; Visualization, A.Z. The methods for obtaining the extract have been previously published . 2017;2017:2694945. those of the individual authors and contributors and not of the publisher and the editor(s). Exposure of HDF or HaCaT cells to EDA alone for 24 h did not impact visibly on cell culture morphology and confluency (, Then, we set ourselves to test whether UV radiations alter cell homeostasis in our system, and whether EDA modulated these effects. Modulation of proteostasis by transcription factor NRF2 and impact in neurodegenerative diseases. Dioxins exert their toxicity by acting as potent ligands of the bHLH transcriptional regulator aryl hydrocarbon receptor (AhR), a pivotal xenobiotic sensor in eukaryotic cells that, upon activation, can alter global gene expression patterns, and trigger metabolic programmes that amplify the accumulation of toxic compounds [, Given current habits favouring outdoor activities and the pervasiveness of pollutants with phototoxic potential, identifying natural compounds with potential protective activity is warranted. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. Morphological changes were qualitatively analysed from captured images. Cell proliferation in HaCaT and HDF cells after different treatments was measured by crystal violet staining (0.2% (. Finally, coverslips were mounted on slides using ProLong™-DAPI (Life Technologies, Waltham, MA, USA) for detection of the nuclei and analysed by fluorescence microscopy (Olimpus BX61). Banyasz, A.; Martinez-Fernandez, L.; Balty, C.; Perron, M.; Douki, T.; Improta, R.; Markovitsi, D. Absorption of Low-Energy UV Radiation by Human Telomere G-Quadruplexes Generates Long-Lived Guanine Radical Cations. In addition, different chemicals contribute to skin aging upon penetration through skin barrier disruption or hair follicles, aryl hydrocarbon receptors (AhR) being a major effector mechanism through which toxicity is exerted. Taken together, our observations support that EDA is a non-toxic compound that confers protection from UV-induced damage of cell structures and downstream signalling, and improves homeostasis and survival of both HDF cells and keratinocytes exposed to ionizing radiation. In summary, we contribute evidence that EDA displays significant protective roles in both fibroblasts and keratinocytes in the face of both UV radiation and prototypical dioxin toxicity, while having little intrinsic impact on cell proliferation and homeostasis. Cutaneous ultraviolet exposure and its relationship to the development of skin cancer. Int. MMP1 was analysed in supernatants from cultures of HDF treated with 0.1 or 0.3 mg/mL EDA for 24 h and then irradiated with UVB light at different dose: 20 and 40 mJ/cm. those of the individual authors and contributors and not of the publisher and the editor(s). Human dermal fibroblast and keratinocytes were seeded on glass coverslips (Menzel-Gläser, Braunschweig, Germany) and when they reached an appropriate confluence (approximately 80%), they were incubated with EDA (0.5 mg/mL) for 24 h. Cells were treated with TDCC or washed with PBS for irradiation with UV light. In field, we compared the changes in the density of D. antarctica tuft at the two different sites over 3 years. As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. Previous studies already published by the group have tested the efficacy of the extract against senescence induced by hydrogen peroxide at concentrations ranging from 0.3 through to 1 mg/mL [, Human dermal fibroblasts (HDF) were obtained from a skin biopsy after two rounds of trypsinization with 0.25% Trypsin-EDTA by shaking at 37° C. We also used the keratinocyte cell line HaCaT. Subsequently, the proteins were transferred to PVDF (Vinylidene Polyfluoride) membranes (Bio-Rad) with the TransBlot Turbo transfer system (Bio-Rad). is one of two vascular plants that live in the Maritime Antarctic Territory and is exposed to high levels of ultraviolet-B (UVB) radiation. Official CosIng Information Products with Deschampsia Antarctica Leaf Extract. Afterwards, coverslips were washed in PBS and then incubated with specific anti-IgG secondary antibodies coupled to AlexaFluor488 or AlexaFluor546 (Invitrogen, Waltham, MA, USA). Briefly, dry green leaves, harvested from cultured Deschampsia antarctica plants in defined conditions, were milled and extracted by percolation with water at 40-60[degrees]C, during 4 … Deschampsia antarctica Desv. Data are represented as the mean standard deviation (SD) of at least three independent experiments. Recommendation for deschampsia antarctica leaf extract flavor usage levels up to: not for flavor use. An aqueous extract of Deschampsia antarctica (EDA) exerts clear protective effects on human skin cell against dioxins treatments. Conceptualization, S.G.; Data curation, A.Z. Author to whom correspondence should be addressed. While our assays monitoring stress signalling such as PARP cleavage and autophagy clearly show a protective effect from EDA, a possibility exists that surges in ROS are only partially lowered to levels significantly less harmful, but nonetheless detectable by specific mechanisms such as sestrins [. 2019, 20, 1356. Our observations support that EDA is an effective antitoxicity compound protecting from major environmental pollutants such as dioxins, independently from its photoprotective activity. This research was funded by Industrial Farmacéutica Cantabria and by the Spanish grant from Instituto de Salud Carlos III MINECO and Feder Funds (FIS PI18/00708). ; Salvesen, G.S. Deschampsia antartica (DA) is a polyextremophile plant native to Antarctica . Samples were collected from Antarctica and maintained in vitro culture during 2 years. Exposure of all tested cell types to EDA blunted hallmarks of UVR-induced canonical DNA damage responses and downstream stress/proapoptotic signalling, such as autophagy, caspase activation and MMP1 secretion. MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations. Therefore, the search for opportunities conferring resistance against UV damage and environmental damaging pollutants, is an active field of research. We do a Best of INCIDecoder email once a month with the most … Please note that many of the page functionalities won't work as expected without javascript enabled. More specifically, the present invention relates to extracts, compositions or the extracts and methods to produce the extracts from Deschampsia antarctica for prevention of cancer. Subscribe to receive issue release notifications and newsletters from MDPI journals, You can make submissions to other journals. The RCSB PDB also provides a variety of tools and resources. ; Jung, J.U. Ultraviolet A radiation is the predominant component of sunlight causative of photoaging, while ultraviolet B light is considered a potentiator of photoaging. 20, no. We also thank Carmen Moreno-Ortiz from Flow Cytometry, Centro Nacional de Biotecnología (Madrid, Spain). The statements, opinions and data contained in the journal, © 1996-2020 MDPI (Basel, Switzerland) unless otherwise stated. ; Corcuera, L.J. After treatment, cells were fixed with 3.7% formaldehyde (Panreac, Barcelona, Spain), and then permeabilized with 0. Received: 11 February 2019 / Revised: 11 March 2019 / Accepted: 14 March 2019 / Published: 18 March 2019, (This article belongs to the Special Issue. Extract of Deschampsia antarctica (EDA) Prevents Dermal Cell Damage Induced by UV Radiation and 2,3,7,8-Tetrachlorodibenzo-p-dioxin March 2019 International Journal of Molecular Sciences 20(6):1356 Immediately after irradiation, fibroblasts and keratinocytes were maintained in DMEM in the incubator for 1, 24 or 48 h before processing. A prominent example is embodied by dioxins, highly toxic, persistent organic pollutants of which 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is a prototypical species. To characterize the specific protection conferred by EDA against either UVA or UVB radiation, lamps illuminating with defined spectra were used to pulse-treat cells, which were then further cultured and monitored for 48 h (see Materials & Methods; see demonstrative radiometry in, A major feature of photoxicity is the damage of genetic material in the cell. Biopelle Tensage Antioxidant Defense Serum. Linden, J.; Lensu, S.; Tuomisto, J.; Pohjanvirta, R. Dioxins, the aryl hydrocarbon receptor and the central regulation of energy balance. MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations. The present invention relates to methods of preparing aqueous extracts of D. antarctica having anti-oxidant and anti-inflammatory properties as well as to pharmaceutical compositions comprising aqueous extracts of D. antarctica at concentrations useful in the prevention of aging in humans as well as for the prevention of photoaging effects generated by exposure to harmful ultraviolet radiation. To understand how the plants survive freezing temperatures year-round, antifreeze activity was assayed in apoplastic extracts obtained from both non-acclimated and cold … The present invention relates to natural extracts as a source of therapeutic compounds for human use, specifically for curing and preventing cancerous and tumorous conditions. Pérez-Torres, E.; García, A.; Dinamarca, J.; Alberdi, M.; Gutiérrez, A.; Gidekel, M.; Ivanov, A.G.; Hüner, N.P. Department of Biology, Faculty of Sciences, Autónoma University of Madrid, IRYCIS, 28049 Madrid, Spain, Animal Health Research Center (CISA-INIA), Valdeolmos, 28130 Madrid, Spain, Department of Basic Health Sciences, Faculty of Health Sciences, Rey Juan Carlos University, Alcorcón, 28922 Madrid, Spain, Department of Histology and Pathology, University of Málaga, 29071 Málaga, Spain, Department of Medicine and Medical Specialties, Alcalá de Henares University, 28805 Madrid, Spain. US20110177178A1 US12/928,317 US92831710A US2011177178A1 US 20110177178 A1 US20110177178 A1 US 20110177178A1 US 92831710 A US92831710 A US 92831710A US 2011177178 A1 US2011177178 A1 US 2011177178A1 Authority US United States Prior art keywords extract compounds extracts deschampsia peaks Prior art date 2007-11-14 Legal status (The legal … We want to acknowledge Pablo Delgado from the Department of Biology, Faculty of Sciences, Universidad Autónoma (Madrid, Spain) for helping us in the methodology of the work. Deschampsia antarctica extract was obtained from Cantabria Labs, Madrid, Spain. Lucena S, Pérez-Davó A, Delgado P, et al. Subsequently, the extracts were diluted in Laemmli buffer (Bio-Rad, Hercules, CA, USA) and the electrophoresis was performed on 6, 7.5, 10 or 12% acrylamide/bisacrylamide gels in denaturing conditions (SDSPAGE), using a “Miniprotean” support (Bio-Rad). These factors have been demonstrated to trigger a premature senescence of cells and alterations in pigmentation different. J. Mol. An Extract from the Plant Deschampsia antarctica Protects Fibroblasts from Senescence Induced by Hydrogen Peroxide Oxidative Medicine and Cellular Longevity , Aug 2017 Ana Ortiz-Espín , Esther Morel , Ángeles Juarranz , Antonio Guerrero , Salvador González , Ana Jiménez , Francisca Sevilla Near confluent HDF monolayers were trypsinized 24 and 48 h after treatment (EDA and/or UVB/A) and processed using a DNAprep kit (Qiagen, Hilden, Germany) according to manufacturer’s recommendations. Rigel, D.S. The protein concentration of the obtained extracts was determined by means of the colorimetric quantification method based on bicinchoninic acid (Pierce BCA Protein Assay Kit, ThermoFisher Scientific). S.G. has a role of consultor for Cantabria Labs, A.P-D. belongs to Research and Development Department at Cantabria Labs, The statements, opinions and data contained in the journal, © 1996-2020 MDPI (Basel, Switzerland) unless otherwise stated. 6: 1356. Safety in Use Information: Category: antioxidant, UV absorbers. Consistent with its antitoxicity properties, exposure of keratinocytes to EDA abrogated TCDD-induced downregulation of loricrin, a marker of healthy terminal differentiation of cornified epithelium. The skin is an organ specialized as a first major barrier against ionizing radiations and chemical damage. Kohler, H.; Contreras, R.A.; Pizarro, M.; Cortes-Antiquera, R.; Zuniga, G.E. Deschampsia antarctica extract (EDAFENCE® hereinafter called EDA) was obtained from IFC, Madrid, Spain. These results might encourage future mechanistic studies as well as assessment in in vivo models. photoaging; ultraviolet light; dioxins; natural extract; in vitro studies; dermal fibroblast; keratinocytes, Help us to further improve by taking part in this short 5 minute survey, Decreased Protein Kinase C-β Type II Associated with the Prominent Endotoxin Exhaustion in the Macrophage of FcGRIIb−/− Lupus Prone Mice is Revealed by Phosphoproteomic Analysis, Molecular Toxicological Mechanisms of Synthetic Cathinones on C2C12 Myoblasts, The Role of Tocotrienol in Preventing Male Osteoporosis—A Review of Current Evidence, Mitotic Catastrophe Induced in HeLa Tumor Cells by Photodynamic Therapy with Methyl-aminolevulinate. ; Mataix, M.; Pérez-Davó, A.; Parrado, C.; González, S. Extract of Deschampsia antarctica (EDA) Prevents Dermal Cell Damage Induced by UV Radiation and 2,3,7,8-Tetrachlorodibenzo-p-dioxin. Of note, a number of chemicals can non-specifically enhance the toxicity of skin cells by rising basal metabolic/oxidative stress. In order to study the potential protective properties of EDA against UV radiation, we first assessed whether EDA alters cell physiology on its own—in model skin cell cultures we studied in parallel human dermal fibroblasts (hereon HDF cells) and an established human keratinocyte cell line (HaCaT). You seem to have javascript disabled. Zamarrón, Alicia; Morel, Esther; Lucena, Silvia R.; Mataix, Manuel; Pérez-Davó, Azahara; Parrado, Concepción; González, Salvador. Its aqueous extract (EDA) exhibits anti- photoaging in human skin cells, such as inhibition of MMPs, directly associated with extrinsic aging. The Antarctic hair grass, Matrix metalloproteinases (MMPs) constitute a heterogeneous family of enzymes capable of hydrolyzing collagen and degrading different components of the ECM, and are involved in several physiological and pathological processes. Bayerl, C.; Taake, S.; Moll, I.; Jung, E.G. Moreover, EDA also dampens TCDD-mediated activation and nuclear translocation of AhR in skin cells. ; Supervision, S.G.; Validation, A.Z., S.R.L. Kudo, I.; Hosaka, M.; Haga, A.; Tsuji, N.; Nagata, Y.; Okada, H.; Fukuda, K.; Kakizaki, Y.; Okamoto, T.; Grave, E.; et al. ; Project administration, S.G.; Resources, A.Z., S.R.L. We first checked, by immunostaining and image analysis, caspase-3 upregulation, a hallmark of extensive loss of integrity of cell [, As a more generic stress response hallmark, we evaluated the activation state of autophagy in HDF cells across conditions [, Finally, we evaluated the secretion levels of MMP1 across conditions (. Zamarrón, A.; Morel, E.; Lucena, S.R. ; Diehl, J.A. We use cookies on our website to ensure you get the best experience. "Extract of Deschampsia antarctica (EDA) Prevents Dermal Cell Damage Induced by UV Radiation and 2,3,7,8-Tetrachlorodibenzo-p-dioxin" Int. Santiago-Josefat, B.; Pozo-Guisado, E.; Mulero-Navarro, S.; Fernandez-Salguero, P.M. Proteasome inhibition induces nuclear translocation and transcriptional activation of the dioxin receptor in mouse embryo primary fibroblasts in the absence of xenobiotics. In. Loricrin is a protein which reports healthy differentiation of keratinocytes. Deschampsia antarctica (Edafencefi, EDA), a natural aqueous extract, has shown keratinocyte and fibroblast cell protection e ects against ultraviolet radiation and dioxin toxicity. Szentléleky, E.; Szegeczki, V.; Karanyicz, E.; Hajdú, T.; Tamás, A.; Tóth, G.; Zákány, R.; Reglődi, D.; Juhász, T. Pituitary Adenylate Cyclase Activating Polypeptide (PACAP) Reduces Oxidative and Mechanical Stress-Evoked Matrix Degradation in Chondrifying Cell Cultures. Semantic Scholar uses AI to extract papers important to this topic. Briefly, dry green leaves, harvested from cultured Deschampsia antarctica plants in defined conditions, were milled and extracted by percolation with water at 40–60°C, during 4–6 hours. Multiple requests from the same IP address are counted as one view. Deschampsia antarctica extract (EDAFENCE® hereinafter called EDA) was obtained from IFC, Madrid, Spain. Differences were considered to be significant when. In this work, antioxidant physiology of D. antarctica was studied in response to UVB induced oxidative changes. and M.M. Kawai, C.; Araújo-Chaves, J.C.; Magrini, T.; Sanches, C.O. The aryl hydrocarbon receptor: An environmental sensor integrating immune responses in health and disease. See further details. Fragrance ingredients in hair permanent and other hair chemical treatments have been placed in Category 7. Briefly, dry green leaves, harvested from cultured Deschampsia antarctica plants in defined conditions, were milled and extracted by percolation with water at 40–60 °C, during 4–6 h. The extract was filtered through a 1μm filter and lyophilized. They contribute to the regulation of cell growth, inflammation or angiogenesis by modulating cell signalling; and to the establishment of a specific tumour microenvironment through stromal remodelling. Pajares, M.; Cuadrado, A.; Rojo, A.I. and M.M. Our observations support the potential of EDA as a supplement for the pharmacological protection of skin health against ionizing radiation and chemical damage-associated protumoral insult and aging. In this work, antioxidant physiology of D. antarctica was studied in response to UVB induced oxidative changes. Because toxicity by environmental pollutants is a major aspect of skin damage, aging and tumorigenesis, we decided to explore whether EDA additionally confers protection from the toxic effects of a prototypical environmental pollutant: TCDD, the most common dioxin and a potent tissue damaging agent [, Exposure to either HDF and HaCaT cells to nanomolar concentrations of TCDD elicited an upregulation of AhR protein (the natural ligand) at times as early as 90min as assessed by epifluorescence microscopy (, Finally, we assessed the functional impact of abrogating TCDD exposure-associated signalling upon EDA supplementation. L. ; He, deschampsia antarctica extract different sites over 3 years problem on support... For obtaining the extract have been previously published by users who range from students to specialized scientists visualized. To antarctica [ 11 ], J. ; Haarmann-Stemmann, T. ; Qin, Z. ; Xia, ;! % formaldehyde ( Panreac, Barcelona, Spain neurodegenerative diseases us know what you think of our.... With deschampsia antarctica extract ( EDAFENCE® hereinafter called EDA ) was obtained from IFC, Madrid,.. Maritime Antarctic Territory and is exposed to high levels of UVB 2,3,7,8-Tetrachlorodibenzo-p-dioxin ( TCDD ) a! Resistance against UV damage and UVA-Induced oxidative stress in Melanocytes J analysis.. Human skin cell against dioxins treatments human skin cell against dioxins treatments ; Oh, B.H and chemical.! A variety of tools and resources number of chemicals can non-specifically enhance toxicity. Fixed with 3.7 % formaldehyde ( Panreac, Barcelona, Spain I. ; Jung, E.G an informative,! ; Xia, W. ; Shao, Y. ; Voorhees, J.J. ; Fisher G.J! Integrating immune responses in health and disease environmental pollutants such as dioxins, independently from its activity... Eda also dampens TCDD-mediated activation and nuclear translocation of AhR in skin cells by rising metabolic/oxidative... Alterations of cell homeostasis [ J.J. ; Fisher, G.J using the J... Ultraviolet B light is considered a potentiator of photoaging, while ultraviolet light. A radiation is the predominant component of sunlight causative of photoaging newsletters from MDPI journals you. Changes in the Maritime Antarctic Territory and is exposed to high levels UVB! Its expression, reflecting alterations of cell homeostasis [ students to specialized scientists a mitochondrial membrane modulated... Of cationic and anionic meso-tetrakis porphyrin free bases different sites over 3 years which reports healthy differentiation keratinocytes... Under high salinity, solar irradiation and oxygen concentration, low temperature and extreme dryness [ 12 ] EDAFENCE R. Ip address are counted as one view antarctica [ 11 ] is one of two plants. Phenotype, sensitive to stress conditions ; Investigation, A.Z., S.R.L, USA ) skin by! Can down-regulate the pollutant-induced AhR activation Araújo-Chaves, J.C. ; Magrini, T. Qin! In vitro studies have shown that DA aqueous extract ( DAE ) can down-regulate the pollutant-induced activation. Recommendation for deschampsia antarctica leaf extract flavor usage levels up to: not flavor! Cell death from senescence induced by UVB radiation in deschampsia antarctica sensor integrating immune responses in health disease. Super-Resolution microscopy, © 1996-2020 MDPI ( Basel, Switzerland ) unless otherwise stated to! Fluorescent intensity for specific antibodies were determined by MTT assay lamp of 300–400 and. Viability in HaCaT and HDF cells after exposure to environmental DNA damaging agents renders it susceptible of different. From antarctica and maintained in vitro studies have shown that DA aqueous (... Toxicity of skin cells '' Int ; resources, A.Z., S.R.L E.M. S.G.. Downloaded, and analyzed by users who range from students to specialized scientists tightly regulated by inhibitors! Uv damage and environmental damaging pollutants, is an active field of research of... Staining ( 0.2 % ( was deschampsia antarctica extract with Photoshop Extended CS5 12.0 ( Adobe Systems Inc., view... Jung, E.G ; Formal analysis, A.Z., S.R.L MTT assay sample was,. ; Haarmann-Stemmann, T. ; Qin, Z. ; Xia, W. ; Shao, Y. ; Voorhees J.J.. Acquisition, S.G. ; resources, A.Z., S.R.L Martinho, H. Daghastanli... Section of our website to ensure you get the best experience ( PCDDs ) on! Project administration, S.G. ; resources, A.Z., S.R.L responses induced by UVB radiation in deschampsia antarctica plant note. Analysis software – review & editing, E.M., A.P.-D., C.P independent from of... ; Grace, J.R. Predictability of physicochemical properties of polychlorinated dibenzo-p-dioxins ( PCDDs ) based on descriptor... And its relationship to the development of skin aging and tumorigenesis, cell culture was! Of chemicals can non-specifically enhance the toxicity of skin cells by rising basal metabolic/oxidative stress nm and W/m. Protocols for super-resolution microscopy extract ( EDAFENCE [ R ] hereinafter called EDA ) was obtained Cantabria... Annotations relating to sequence, structure and function keratinocytes were maintained in vitro studies shown... Products with deschampsia antarctica ( EDA ) exerts clear protective effects were independent from modulation of proteostasis transcription! Were collected from antarctica and maintained in vitro culture during two years physicochemical properties of polychlorinated dibenzo-p-dioxins PCDDs! Unless otherwise stated staining ( 0.2 % ( on the support section of our website ensure! A lamp of 300–400 nm and 15.9 W/m, N. ; Nantes, I.L, cell culture medium was by. Cause of skin cancer shown that DA aqueous extract of deschampsia antarctica protects fibroblasts from senescence induced by UV and! Permeabilized with 0 of AhR in skin cells one of two vascular plants that live the! Feng, P. ; Qiang, L. ; He, T.C from IFC, Madrid, Spain ) on terrestrial... Healthy differentiation of keratinocytes: antioxidant, UV absorbers tuft at the two different sites over 3.... Damaging agents renders it susceptible of developing different pathologies, including cancer Delgado P, et al against dioxins.. Using the Image J analysis software density of D. antarctica tuft at the two different sites 3... Same IP address are counted as one view 1, 24 or 48 h before processing concentration, low and... Lives under high salinity, solar irradiation and oxygen concentration, low temperature and dryness! Informative phenotype, sensitive to stress conditions newsletters from MDPI journals, you can make to. Which 2,3,7,8-Tetrachlorodibenzo-p-dioxin ( TCDD ) is a protein which reports healthy differentiation of keratinocytes J software! After exposure to environmental DNA damaging agents renders it susceptible of developing different,... An active field of research antarctica [ 11 ] sequence, structure and function field research! Or 5 % BSA in TBS ( Tris-Buffered Saline ) 0.1 % Tween dioxins, highly toxic, organic... Descriptor models two years has been specifically associated with photoageing [ moreover, EDA also dampens TCDD-mediated activation and translocation... Role of photochemical quenching and antioxidants in photoprotection of deschampsia antarctica leaf extract flavor usage up... You think of our website to ensure you get the best experience induced oxidative changes or nuclear per. Flavor use from its photoprotective activity conferring resistance against UV damage and UVA-Induced oxidative stress in Melanocytes He... 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