Learn vocabulary, terms, and more with flashcards, games, and other study tools. Click to see … While most micro-organisms are not pathogenic, in the microbiology laboratory we assume that all micro-organisms are potentially pathogenic and act accordingly. The magnitude of the effect of handwashing is mainly a function of wash time and soap usage. Dec. 8, 2020. Environment 5.1 Premises Microbiology laboratories should be separated from production areas and have an air handling system separated from the production areas. I was the one who had to do the washing so I did it, and then we swabbed my hands with sterile swabs and streaked it on some TSA agar plates. Rub hands together rapidly across all surfaces of your hands and wrists to help remove dirt and germs. Why do you think hand washing is necessary when medical and surgical personnel wear gloves during surgery or when examining patients? . Student 2 proceeds to wash for 3 minutes the thumb of the right hand under a stream of warm running water using a scrubbing motion between the thumb and the right index finger.  Student 1 then proceeds to run a sterile cotton swab on the pad of the thumb of Student 2 and then inoculates the R4 section of the “Water” plate using a zigzag motion. We tested handwashing techniques with a simple experiment in our Microbiology Lab at Hamilton General Hospital. How does the presence of residential flora influence the infectious process? Lesson Type: Experiment and Extension Lesson—Students collect, manipulate, and/or summarize data from … Owensboro Community and Technical College, University of Illinois, Urbana Champaign • MCB 251, Owensboro Community and Technical College • BIO 225, University of North Georgia, Gainesville • BIO 2500. Your lab coat (will be provided to you by Valencia)-must be … Apply one dose of liquid soap and lather (wash) well for 15–20 seconds (or longer if the dirt is ingrained). Correct hand-washing is the single most effective technique that prevents the transmission of micro-organisms either directly or indirectly to others. . Wash thoroughly with your washing agent for two (2) minutes. They learn to create a … Figure 1.2 Agar plates inoculated with microorganisms from the left hand thumb. I (Lauren) joined the team just before the … . Two hand-washing methods, using hot and cold water with soap, were tested through colonial growth observation to determine how successful they were in the removal of bacteria. The term “hand hygiene” represents a new term in the healthcare vocabulary, replacing the more narrow term of “handwashing”. Figure 1.1 Agar plates inoculated with microorganisms from the right hand thumb. (cdc.gov). Lab Exercise 1-1 (Proper Hand-Washing Technique).....6 Lab Exercise 1-2 (Bacteria are Ubiquitous) ... Report any injury to your instructor. Resident flora are the normal flora of the skin. After washing, rinse thoroughly with tap water. The benefits of liquid soap include: Hygiene – it … The results from the hand washing experiment indicate that washing with water is effective.  Also, washing with antibacterial soap appeared to be effective also but initially lagged in removing microorganisms.  One reason for this lag may be that the soap was effective in loosening the microorganisms at deeper layers of the epidermis.  The results from this experiment are somewhat misleading since there is documented evidence that the best methods for infection control involve the use of antimicrobial agents (Kampf).  There is also evidence that “a hygienic hand disinfection with an alcohol-based hand rub is the preferred treatment to be carried out after patient care activities that could lead to contamination of the hands of the health care workers” (Kamp). Wash your hands with disinfectant soap when you arrive at the lab and again before you leave. They would be in the Results section. The purpose of this manual is to provide teachers and technicians with good techniques in practical microbiology to ensure that investigations proceed safely and achieve the required educational aims … Lather your hands both front and back including the fingers and nails, by rubbing them together with the soap. Your email address will not be published. Your hands should be wet throughout the washing. ehp Handwashing 101 LESSON: Summary: Students develop a hypothesis about the most effective handwashing method, develop an experiment protocol to test their hypothesis, and carry out their handwashing experiment. Below are the introduction, body and conclusion parts of this essay. Hand hygiene is a comprehensive term that refers to handwashing, hand antisepsis and actions taken to maintain healthy hands and fingernails. Course Hero is not sponsored or endorsed by any college or university. Analysis of total lab automation instruments finds limited risk from processing specimens, but the lab environment faces exposure from staff’s handling of samples, especially without strict adherence to safety protocols. Prezi Video + Unsplash: Access over two million images to tell your story through video “Infection control in dental settings,” cdc.gov.  Centers for disease control and prevention. The procedure consists of the following steps.  Using the glass marking pencil, Student 1 marks the bottom of two agar plates with the label “Water” and the other two plates with the label “Soap”.  The Student 1 then marks the bottom of each “Water” and “Soap” plate with a dividing middle line.  Each half of a “Water” plate is labeled R1 and R2 respectively.  Each half of the other “Water” plate is labeled R3 and R4 respectively.  Each half of a “Soap” plate is labeled L1 and L2 respectively.  Each half of the other “Soap” plate is labeled L3 and L4 respectively. Your email address will not be published. Cultural Characteristics of Microorganisms, Identification of Human Streptococcal Pathogens, Use of Differential, Selective, and Enriched Media, Growth(0 = none,1+ = slight,2+ = moderate,3+ = heavy,4+ = maximum). Date: SEP.17.2020 // … . This essay sample on Microbiology Lab Report Example provides all necessary basic info on this matter, including the most common “for and against” arguments. Student 2 proceeds to wash for one minute the thumb of the right hand under a stream of warm running water using a scrubbing motion between the thumb and the right index finger.  Student 1 then proceeds to rub a sterile cotton swab on the pad of the thumb of Student 2 and then inoculates the R2 section of the “Water” plate using a zigzag motion. Our 2020 Prezi Staff Picks: Celebrating a year of incredible Prezi videos; Dec. 1, 2020. We hypothesized that the plate inoculated with a clean fingertip would present with fewer bacterial colonies than those inoculated with an unwashed fingertip. Generally, it is better to use liquid soap than bar soap, particularly at work. The three main elements of safe containment of microorganisms are (1) good laboratory practices and technique, (2) safety equipment, and (3) facility design. Microbiology studies microscopic spoilage organisms such as bacteria, viruses and pathogens. Rub your hands together to make a lather and scrub them well. 3. Continue rubbing your hands for at least 20 seconds. Web. 2. Hand Washing Lab Report - Hand Washing Effectiveness In Cold Vs Hot Water Adele Weston Introduction Hand washing is an important method of prevention, 21 out of 25 people found this document helpful, Hand Washing Effectiveness In Cold Vs. is more effective in reducing bacteria when used with. C. EFFECTIVENESS OF HAND WASHING . Dry your hands using a clean towel or air dry them; Note: Use soap or alcohol-based hand sanitizer to get rid of … . To clean our hands, the Centers for Disease Control and Prevention recommends that we: wet hands with clean water. Blog. 5 April 2014. Vigorous hand washing… Follow Published on Oct 20 ... follow the above rules will be sent out of the class. Create a free website or blog at WordPress.com. This laboratory exercise illustrates the critical need for proper hand washing techniques as a means of reducing the incidence of healthcare facility acquired infections. why did state or three sentences, In two Background … Kampf, Gunter. According to research literature, hand washing with water only is ineffective.  Using soap for extended periods of time is a better alternative to using only water.  However, this allows some transient flora to cling deeply to hair and to remain protected by oil and dead skin cells.  Soap with surgical scrubbing is a good way to remove transient flora and some residential flora. Purpose: In this lab, you are going to assess the value of that hand-washing advice and the methods that bring the most benefit from hand washing. Effectiveness from hand sanitizers is best when a large volume of product is applied to the hands. good laboratory practices and technique, (2) safety equipment, and (3) facility design. Transient flora are the microorganisms you pick up from what you have been handling. Before birth, the skin of a human being is sterile.  During the birthing process and subsequently, the human skin is colonized by microorganisms.  The set of microorganisms that permanently colonize the human skin and do not cause harm to a healthy individual is called the residential flora.  The set of microorganisms that are present only temporarily on the skin is called the transient flora.  The residential flora tends to be located in the deeper layers of the epidermis and in the hair follicles.  The transient flora is located superficially.  The residential flora is more difficult to remove from the skin than the transient flora.  The residential flora exists in a symbiotic relationship with the host.  By competing for resources with pathogenic microorganisms, the resident flora can provide benefit to the host (Cappuccino 7). It is routine practice to wash the hands prior to and after examining a … . In addition to hand bacteria, hands were inoculated with, Results of the hand-washing test showed that a thorough, cleansing of the hands with soap and water reduces bacterial numbers and therefore. Purpose: The purpose of this experiment is to test the effectiveness of washing and drying hands on the number of bacteria on the hands. Wet your hands with warm water. All healthcare providers ultimately have to take a microbiology class as part of their program curriculum. Scrub your hands for at least 20 seconds. We tested handwashing techniques with a simple experiment in our Microbiology Lab at Hamilton General Hospital. Purpose: In this lab, you are going to assess the value of that hand-washing advice and the methods that bring the most benefit from hand washing. sections: following Purpose you the experiment. Student 2 proceeds to add a drop of antibacterial soap to the pad of his left hand thumb and then wash for one minute the thumb under a stream of warm running water using a scrubbing motion between the thumb and the left index finger.  Student 1 then proceeds to rub a sterile cotton swab on the pad of the thumb of Student 2 and then inoculates the L2 section of the “Soap” plate using a zigzag motion. Thoroughly washing hands is the single most important thing students can do to keep from getting sick, or to keep from infecting others. We printed our fingers in petri dishes before hand washing, after poor hand washing, and after proper hand washing, then incubated them overnight to allow the bacteria to grow. This is my video submission for the Hand Washing Lab for my Microbiology class. Residential flora is difficult to remove with simple hand washing.  Transient flora is easier to remove.  Surgical scrubbing is the best way to remove transient flora and reduce the presence of residential flora. The agar plates were visually examined after the period of incubation and the observations appear in Table 1.1.  The growth for the “Water” plates starts heavy and progressively diminishes to slight growth.  The growth for the “Soap” plates starts at heavy and also diminishes to slight growth but shows lag in the rate of decrease at the beginning. be typed and report must experiment. Student 1 uses aseptic technique to soak a sterile cotton swab in sterile saline solution in a test tube.  The aseptic technique consists of removing the cap of the test tube, flaming the neck of the test tube using the Bunsen burner, dipping the cotton swab in saline, and putting the cap back on the test tube.  Student 1 then proceeds to rub the moistened cotton swab on the pad of the right hand thumb of Student 2 and then inoculates the R1 section of the “Water” plate using a zigzag pattern from the edge of the dish towards the dividing middle line. Hand Washing Video by CDC The typical person’s hands contain millions of microbes. Student 2 proceeds to add a drop of antibacterial soap to the pad of his left hand thumb and then wash for 3 minutes the thumb under a stream of warm running water using a scrubbing motion between the thumb and the left index finger.  Student 1 then proceeds to rub a sterile cotton swab on the pad of the thumb of Student 2 and then inoculates the L4 section of the “Soap” plate using a zigzag motion. Microbiology Lab Practices and Safety Rules 1. 3. 1 Hand washing is a simple, convenient, effective, and cost-effective means to limit the transmission of communicable diseases from one person to another. This experiment will basically test whether bacteria are better cleaned using soap, drying with paper towels, air drying, or using chemicals like hydrogen peroxide or alcohol Figure 1.1 shows an image of the agar plates for the right hand.  Figure 1.2 shows an image of the agar plates for the left hand. Involvement of several members of our research team in the Work Group led to a great opportunity to develop a research collaboration with an acute care hospital.             Table 1.2 Percent microorganism growth reduction. Student 1 uses aseptic technique to soak a sterile cotton swab in sterile saline solution in a test tube.  Student 1 then proceeds to rub the moistened cotton swab on the pad of the left hand thumb of Student 2 and then inoculates the L1 section of the “Soap” plate using a zigzag pattern from the edge of the dish towards the dividing middle line. Boston: Pearson, 2014. Hand Wash Lab Introduction: You are aware that you have been taught to wash your hands often, especially before eating and after; well, you know. 4. Do not put This experiment asks them to test antimicrobial chemicals for use in soaps. Washing hands without soap is much less effective. . Most are naturally occurring and are harmless, but some may be disease-causing germs. Microbiology 1. Be sure to scrub the backs of your hands, between your fingers, and under your nails. Rinse your hands well under clean, running water. After checking on it a week later, a ton of … Scrub your hands for at least 20 seconds. Tenth Edition. Table 1.1 Macroscopic observations of microorganism growth. Liquid soap is better than bar soap, especially at work. 2. There are 2 categories of microorganisms, or flora, normally found on the hands. The materials for this laboratory experiment include: 4 nutrient agar plates; liquid antibacterial soap; 8 sterile cotton swabs, 1 test tube of sterile saline; Bunsen burner, glass marking pencil; stopwatch. 5. It's important to have control plates to distinguish between bacteria that possibly came from the substance/object you were using and the resident/transient bacteria on the hand. Hand Hygiene Simplest & Most Effective measure to prevent infection 4. It is necessary to wash hands when wearing gloves during surgery or when examining patients because the gloves can have defects that allow microorganisms to pass through them.  In addition, there is risk that the glove may become damaged during surgery and the patient become exposed to the microorganisms resident on the hands.  It is advisable to perform hand washing after removing gloves to reduce the risk that microorganisms might have been transferred from the glove surface to the hands. These included an active dry yeast packet, distilled water, sugar, hand soap, nutrient agar, wooden stir stick, hot pad, scissors, petri dishes, cotton swabs, sterile gloves, 250 beaker, transfer pipettes, parafilm, … Materials Distilled water Test tube 6 Unopened packages of 1 sterile cotton swab 2 sterile … Analysis of total lab automation instruments finds limited risk from processing specimens, but the lab environment faces exposure from staff’s handling of samples, especially without strict adherence to safety protocols. C. EFFECTIVENESS OF HAND WASHING . HAND HYGIENE 1 2. Wash and dry your hands carefully. This preview shows page 1 - 2 out of 4 pages. as microbiology cannot be achieved effectively without enhancing the theory with “hands on” experience in the laboratory. My group had the test of no soap and washing for periods up to 2 minutes. Applying a large volume of hand sanitizer ensures excess active ingredient and … The exercise compares the effectiveness of two hand washing techniques: using water only, and using soap and water. Cappuccino, James, and Natalie Sherman. Microbiology: A Laboratory Manual. Needed for Each Lab To participate in the lab, you must have the following items: 1. zWash hand immediately after accidental contamination with blood/body fluid, before eating and drinking and after removing gowns/coats and gloves. The experiment should be modified to have a better starting condition for the hand contamination.  Both hands should be used to contact the same surfaces where microorganisms are known to be present.
After the completion of the day's work and before leaving wash your hands with carbolic soap with water. Please note that due to formatting issues the flow charts had to be removed. Hand Washing: Weak Link in Preventing Microbial Contamination in Labs. In our microbiology lab, we did the classic hand washing experiment. Proper hand washing can protect you and others from a range of diseases. Rinse and dry thoroughly. Hand Washing: Weak Link in Preventing Microbial Contamination in Labs. Learn vocabulary, terms, and more with flashcards, games, and other study tools. Microbiology (lab report 1 format) 28,594 views. Ten weeks into the pandemic, handwashing with soap and other public health measures like maintaining physical distance, practising cough etiquette and wearing a mask stay the best defence against coronavirus, the World Health Organisation said Global Handwashing Day is observed on October 15 each year to increase … Bacteria Lab Report . It is essential to know how just effective hand, washing is. apply soap and lather/scrub every nook and cranny of your hands for 20-30 seconds (about the time to sing "Happy Birthday" twice). Student 2 proceeds to wash for 2 minutes the thumb of the right hand under as stream of warm running water using a scrubbing motion between the thumb and the right index finger.  Student 1 then proceeds to rub a sterile cotton swab on the pad of the thumb of Student 2 and then inoculates the R3 section of the “Water” plate using a zigzag motion. Rinse your hands well under clean, running water. 10 July 2013. Resident flora are the normal flora of the skin. This laboratory exercise illustrates the critical need for proper hand washing techniques as a means of reducing the incidence of healthcare facility acquired infections.  The exercise compares the effectiveness of two hand washing techniques: using water only, and using soap and water.  The student is exposed to the fact that the hands harbor microorganisms that could result in transmitting disease.  In addition, the microorganisms resident in the hands could result in contamination of laboratory experiments if aseptic techniques, such as wearing gloves, are not strictly followed. The Effectiveness of Washing Hands. In addition to hand bacteria, hands were inoculated with table surface bacteria. There are 2 categories of microorganisms, or flora, normally found on the hands. Procedure: 1. Required fields are marked *. Proper hand washing at healthcare facilities has the goal of removing the transient flora from the skin on the hands and also to diminish the presence of the permanent flora.  A serious risk of infection exists if these microorganisms are allowed to enter the body tissues. Here is an excellent example of how to write an unknown lab report in Microbiology class. The students investigate the scientific method by a prompt in the form of a letter asking them to design a hand washing experiment. IMPORTANCE OF HAND HYGIENE 3 Materials and Methods Several materials were used in order to conduct this experiment. Hand Hygiene - Prac. We printed our fingers in petri dishes before hand washing, after poor hand washing, and after proper hand washing, then incubated them overnight to allow the bacteria to grow. How does hand washing affect residential versus transient flora? Student 2 proceeds to add a drop of antibacterial soap to the pad of his left hand thumb and then wash for 2 minutes the thumb under a stream of warm running water using a scrubbing motion between the thumb and the left index finger.  Student 1 then proceeds to rub a sterile cotton swab on the pad of the thumb of Student 2 and then inoculates the L3 section of the “Soap” plate using a zigzag motion. The Biofilm Laboratory research team focuses on the role of microbial biofilms in healthcare-associated infections. The results … Cdc.gov. Microbiology laboratories should be designed and have sufficient space to suit the operations … Residential flora is at a deeper layer of the skin as compared with transient flora.  Residential flora is harder to remove than transient flora.  Normally, residential flora exists symbiotically with the skin.  It can present a risk for infection in individuals whose immune system is compromised.  Residential flora can cause infections if they gain entrance to the human tissues. Transient flora are the microorganisms you pick up from what you have been handling. Start studying Hand washing - Microbiology. rinse well with clean running water. The participants in the experiment are Student 1 and Student 2. Microbiology Lab Practices and Safety Rules 1. is an effective measure in combatting pathogenic bacteria. . Compare the effectiveness of hand washing with water, with soap, and with soap and surgical scrubbing. Take two sterile … Web. 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